You've verified that PTFB Pro is seeing the target by using a five second delay and watching for a red border around PTFB Pro's tray icon, but the actual macro/press isn't working...

• First check that your computer protection software (e.g. antivirus, firewall) isn't blocking PTFB Pro's actions.
  
• Also check that there are no "always on top" windows floating above the the target. If there are, they will intercept PTFB Pro's simulated mouse and key presses.
• If the target in question is the "Single Press" type, try recreating it as a Sequence (and vice-versa).
• If the target in question is the Sequence type, try reducing the playback speed, or disable elimination or redundant mouse movement. Also, try recreating the Sequence but start it by clicking in the window to make sure it gets focus early on.
• Likewise, if you're getting PTFB Pro to simulate mouse activities, but the same task can be accomplished via the keyboard, try the keyboard approach, and vice-versa.